Flashcards in Vectors 2:Bacteriophages Deck (6)
How are complete λ bacteriophages formed?
Bacteriophage λ undergoes either lytic replication or lysogeny following the infection of E. coli
Lytic replication results in cell death of E. coli and the formation of clear plaques on a cell lawn
The heads and tails of λ systems are self-assembling proteins
The addition of DNA containing COS sites ate the correct spacing (43-53 kb) allows spontaneous formation of a complete λ phage
Describe λ phage vectors
Have a central stuffer fragment which is replaced by DNA to be cloned
What is the central stuffer?
Contains genes used in the lysogenic life cycle
What type of DNA can be taken up by a λ phage vector?
How does the DNA fragment fit into the vector?
It replaces a part of the phage