Vectors 2:Bacteriophages Flashcards Preview

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Flashcards in Vectors 2:Bacteriophages Deck (6)
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1

How are complete λ bacteriophages formed?

Bacteriophage λ undergoes either lytic replication or lysogeny following the infection of E. coli
Lytic replication results in cell death of E. coli and the formation of clear plaques on a cell lawn
The heads and tails of λ systems are self-assembling proteins
The addition of DNA containing COS sites ate the correct spacing (43-53 kb) allows spontaneous formation of a complete λ phage

2

Describe λ phage vectors

Have a central stuffer fragment which is replaced by DNA to be cloned

3

What is the central stuffer?

Contains genes used in the lysogenic life cycle

4

What type of DNA can be taken up by a λ phage vector?

Linear DNA

5

How does the DNA fragment fit into the vector?

It replaces a part of the phage

6

How efficient is the transformation?

Very efficient (10^9 μ^-1) and is constant with increasing insert size