Flashcards in HIV Deck (16)
1
HIV classification and characteristics:
o Genome: 2 copies of positive polarity RNA
o Capsid symmetry: Icosahedral
o Envelope: Yes; includes gp120 and gp41 (mediate interaction between viral partical and host cell)
o Tropism: CD4+ T cells (also CD4+ monocytes, macrophages)
o Reverse transcriptase + integrase + protease = 3 E that come with the virus
2
capsid protein detectable by ELISA for dx:
p24
3
3 viral enzymes:
Reverse transcriptase
integrase
protease
4
2 envelope glycoproteins:
gp 120
gp 41
5
viral protein that binds CD4:
gp 120
6
viral protein binds chemokine R, initiates fusion with host plasma membrane
gp 41
7
What does reverse transcriptase do?
Where does it do it?
ssRNA --> ssDNA --> dsDNA
cytoplasm
8
Enzyme that gets viral dsDNA into host DNA?
integrase
9
last viral enzyme used to package new viruses?
protease
10
what makes the new viral RNA and mRNA?
host RNA pol 2
11
How is HIV tested for?
ELISA for anti-p24 antibodies
then
confirm with Western Blot
12
which two enzymes are required for formation of the provirus?
reverse transcriptase
integrase
13
major cause of CD4 cell loss in HIV?
binding of viral envelope proteins expressed on infected cell surface binds to CXCR4 --> autophagy
14
co-receptors for viral binding to host cell:
CXCR4
CCR5
15
gag?
capsid proteins
ie. p24
16