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IHO week 6 and Final > HIV > Flashcards

Flashcards in HIV Deck (16):
1

HIV classification and characteristics:

o Genome: 2 copies of positive polarity RNA

o Capsid symmetry: Icosahedral

o Envelope: Yes; includes gp120 and gp41 (mediate interaction between viral partical and host cell)

o Tropism: CD4+ T cells (also CD4+ monocytes, macrophages)

o Reverse transcriptase + integrase + protease = 3 E that come with the virus

2

capsid protein detectable by ELISA for dx:

p24

3

3 viral enzymes:

Reverse transcriptase

integrase

protease

4

2 envelope glycoproteins:

gp 120

gp 41

5

viral protein that binds CD4:

gp 120

6

viral protein binds chemokine R, initiates fusion with host plasma membrane

gp 41

7

What does reverse transcriptase do?

Where does it do it?

ssRNA --> ssDNA --> dsDNA

cytoplasm

8

Enzyme that gets viral dsDNA into host DNA?

integrase

9

last viral enzyme used to package new viruses?

protease

10

what makes the new viral RNA and mRNA?

host RNA pol 2

11

How is HIV tested for?

ELISA for anti-p24 antibodies

then

confirm with Western Blot

12

which two enzymes are required for formation of the provirus?

reverse transcriptase

integrase

13

major cause of CD4 cell loss in HIV?

binding of viral envelope proteins expressed on infected cell surface binds to CXCR4 --> autophagy

14

co-receptors for viral binding to host cell:

CXCR4

CCR5

15

gag?

capsid proteins

ie. p24

16

Mutation leading to resistance to HIV infection?

CCR5