Immunologic testing Flashcards Preview

IHO week 6 and Final > Immunologic testing > Flashcards

Flashcards in Immunologic testing Deck (15):
1

Assay with antigen forming lattice of antibodies--> clumping visible to naked eye:

direct agglutination assay

2

Assay with latex beads bound to Fc of antibodies --> clump if antigen present --> latex beads are what is actually visualized:

passive agglutination assay

3

Binding of antibody and antigen at "equivalence" concentration forms visible precipitate:

Immunoprecipitation Assay

4

Hemagglutination to detect antibodies present, positive test = ?

RBC's float

(negative test sinks to form a "button")

5

Hemagglutination to detect virus, positive test = ?

RBCs sink, form:

BUTTON

6

Negative test result in complement fixation assays:

RBC lysis

7

Fluorescently tagged antibodies bind to antigen:

Direct immnofluorescence

8

Fluorescently tagged antibodies bind to unlabeled anti-antigen antibodies:

Indirect Immunofluorescence

9

Antibodies attached to surface of microtiter well --> antigen binds on top --> tagged antibodies bind on top of antigen --> add substrate for enzyme and measure color present to QUANTIFY amount of antigen present:

Direct ELISA

10

Antigen attached to surface of microtiter --> antibody binds on top --> anti-antibody antibodies bind on top (tagged)

-tells what kind of Ig's present (IgG, IgM, etc)

-also quantitative

Indirect ELISA

11

Antigen attached to microtiter surface --> no reaction = positive result?

Competitive ELISA

12

Gel containing proteins gets electric current passed through

-used to dx HIV

Western Blot

13

Laser looks for surface proteins to identify cell types present?

Flow Cytometry

14

T-cell : all other lympocytes (ratio)

2:1

15

B-cell : NK cell (ratio)

3:1