Flashcards in Immunologic testing Deck (15)
Assay with antigen forming lattice of antibodies--> clumping visible to naked eye:
direct agglutination assay
Assay with latex beads bound to Fc of antibodies --> clump if antigen present --> latex beads are what is actually visualized:
passive agglutination assay
Binding of antibody and antigen at "equivalence" concentration forms visible precipitate:
Hemagglutination to detect antibodies present, positive test = ?
(negative test sinks to form a "button")
Hemagglutination to detect virus, positive test = ?
RBCs sink, form:
Negative test result in complement fixation assays:
Fluorescently tagged antibodies bind to antigen:
Fluorescently tagged antibodies bind to unlabeled anti-antigen antibodies:
Antibodies attached to surface of microtiter well --> antigen binds on top --> tagged antibodies bind on top of antigen --> add substrate for enzyme and measure color present to QUANTIFY amount of antigen present:
Antigen attached to surface of microtiter --> antibody binds on top --> anti-antibody antibodies bind on top (tagged)
-tells what kind of Ig's present (IgG, IgM, etc)
Antigen attached to microtiter surface --> no reaction = positive result?
Gel containing proteins gets electric current passed through
-used to dx HIV
Laser looks for surface proteins to identify cell types present?
T-cell : all other lympocytes (ratio)