Flashcards in Other Gram Negative Rods Deck (56)
0
False about Vibrio cholerae
A.Comma shaped, curved rod
B. Single flagellum & motile
C. Facultative anaerobes
D. Oxidase negative
D. Oxidase negative
Positive dapat
1
False about Vibrio cholerae
A.Tolerate alkaline media
B.Sensitive to acid
C.Maybe halophilic
D. None of those mentioned
D.
2
False about antigenic structure of Vibrio cholerae
A.Single heat stabile H antigen (in several Vibrio)
B.O lipopolysaccharide Found in V.cholerae
C. O LPS Used for serologic specificity
A.Single heat stabile H antigen (in several Vibrio)
Labile dapat
3
Differentiate serotype from biotype
Serotype-classified based on cell surface antigen
Biotype-Classified based on physical and/or biochemical properties
4
Two biotypes of Vibrio cholerae O1 antigen
Classical and El Tor
5
Adherence factors of Vibrio cholerae
hemagglutinin, toxin-coregulated pili)
6
Motility, Mucinolytic enzymes, Cholera exotoxin, Neuraminidase, Proteases
Which is not a virulence factor of V.cholera?
Cholera exotoxin.
Enterotoxin dapat
7
False about Cholera enterotoxin
A.Heat labile (same as LT of ETEC)
B.Composed of subunit A and B
C. Subunit A catalyzes ADP-ribosylation regulator complex
D. Increase in cAMP prolonged hypersecretion of water and electrolytes
E. Stimulates absorption of Na+ and Cl-
E. Stimulates absorption of Na+ and Cl-
Inhibits dapat
8
Which is not true about V.cholera O139?
A.Derived from the pandemic El Tor biotype
B.Lost the characteristic O1 somatic antigen
C.Produces a polysaccharide capsule and a different cholera enterotoxin D.Retained the epidemic potential of O1 strain
C.Produces a polysaccharide capsule and a different cholera enterotoxin
Same enterotoxin pa rin
9
V.cholera onset of symptoms
2-3 days
10
V.cholera mode of transmission
ingestion of contaminated food and water
11
Fill in the blanks(V.cholera clinical manifestations):
Watery diarrhea (___ stools)
Vomiting
Loss of 15-20 liters of fluid/day Dehydration and shock
___ hands
Fluid and electrolyte imbalance
Rice water
Washer woman's
12
True or false: In lab diagnosis of Vibrio cholera, Gram stain is done on stool specimen
False. From plate dapat
13
In lab diagnosis of Vibrio cholera, what kind of smear is done?
dark filed microscopy/phase contrast
14
What specific media is used for Vibrio cholerae?
Thiosulfate Bile Salt Agar
15
Which is false in TCBS Culture?
A. Alkaline peptone water must be applied for 8 hours after culture
B. Should be held for 48 hours before reporting as negative for V. cholerae
C. Both are false
A. Alkaline peptone water must be applied for 8 hours after culture
prior to culture dapat
16
color of colonies on TCBS
___for V. cholerae
__ for V. parahemolyticus
Yellow
Green
17
Vibrio species causing Septicemia, wound infection
V.vulnificus
18
False about Tetracycline
a. Interferes with nucleic acid synthesis
b. Shortens the period of excretion of Vibrio
C. Reduces stool output in cholera
D. Must be given for 3 days to patients with cholera
a. Interferes with nucleic acid synthesis
Protein dapat
19
True or false: Vaccine can be used to prevent cholera
True (oral cholera vaccine)
20
False about Campylobacter jejuni
A. comma, S or ‘gull wing’ shapes
B. Motile with 1 flagellum
C. Aerobic, non-fermenting
D. oxidase positive
C. Aerobic, non-fermenting
Microaerophilic dapat
21
Which is false about C.jejuni?
A.has LPS with endocytic activity
B.parasites in GIT of meat and poultry
C.mode of transmission: ingestion of contaminated food and water
B.parasites in GIT of meat and poultry
Commensals dapat
22
False about Pathology of C.jejuni
A.Multiply in large intestines, invade the epithelium producing inflammation
B.Acute exudative and hemorrhagic inflammation
C.Increase in WBC or pus cells in fecalysis
D.Can invade the bloodstream
E.Heat-labile toxin may cause diarrhea
A.Multiply in large intestines, invade the epithelium producing inflammation
Small dapat
23
For C.jejuni
A.Onset of symptoms: 2-4 days B.Incubation period: 1-4 days C.Symptoms last for 2-4 days
Which is false?
C. 7-10 dapat
24
Which is not a possible complication of C.jejuni infection?
(1) Reactive arthritis
(2) Hemolytic uremic syndrome
(3) Meningitis
(4) Recurrent colitis
(5) Chronic cholecystitis
(5) Chronic cholecystitis
Acute dapat :)
25
Complication of C.jejuni infection involving progressive and ascending paralysis of muscle groups
Guillain-Barre syndrome
26
In lab diagnosis of C.jejuni, which is not done?
A. Gram stain
B. Culture on TCBS
C. Dark field microscopy
B. Culture on TCBS
Skirrow's or Campy blood agar dapat
27
Campy blood agar has the same mechanism as ___ in V.cholera
Alkaline peptone water
28
False about H.pylori
A.Found in esophagus + the mucus gel that coats the stomach epithelium
B.secreted in the poop
C.may be part of normal flora
D.Grows at pH 6.0 – 7.0
B
29
(1) Spiral shaped, gram (-) rods
(2) Motile
(3) Urease (-)
Which is not true about H.pylori?
(3)
Dapat urease positive
30
What does H.pylori produce to neutralize stomach acidity?
NH2
31
Protease is one of H.pylori's virulence factors. What does it do?
modifies gastric mucosa and reduce acidity
32
Two substances that attract H.pylori to gastric mucosa
Hemin and urea
33
False about Culture of gastric biopsy tissue for H.pylori
A.Chocolate agar or TCBS agar
B. Oxidase (+), catalase (+), urease (+)
C. Both are false
A.Chocolate agar or TCBS agar
Skirrow, not TCBS
34
False about treating H.pylori
(1) Bismuth salt + 2 antibiotics
(2) Proton pump stimulator
(3)both are false
(2) Proton pump stimulator
Inhibitor dapat
35
False about Pseudomonas
A. Anaerobic bacilli
B.Motility is by single, polar flagellum
C. more than 140 species are Mostly saprophytic
A. Anaerobic bacilli
Aerobic dapat
36
False about growth requirements of Pseudomonas aeruginosa
(1) Non-fermentative aerobe
(2) Grown on media supplying only acetate for carbon and ammonium sulfate for nitrogen
(3) use sulfate as electron acceptor
(4) Grows best at 25 to 37⁰C, but may survive at lower or higher
(5) Resists high concentrations of salt, dyes, weak antiseptics, and many commonly used antibiotics
(3) use sulfate as electron acceptor
Nitrate dapat
37
Pseudomonas modes of transmissions: which is not part?
(1) Patient to patient on the hands of hospital personnel
(2) Direct patient contact with contaminated reservoirs
(3)Ingestion of contaminated food & water
(4)sexual contact
(4)sexual contact
38
False about P.aeruginosa
A.Pathogenic in areas devoid of normal defenses
B.non-invasive
C.toxigenic
B
Invasive siya
39
What is alginate (in P.aeruginosa)?
exopolysaccharide that produces biofilm
40
Alginate is seen in __ fibrosis
Cystic
41
Toxin A of Pseudomonas is similar to ___ toxin
Diptheria
42
Color of pus in burn infections caused by P.aeruginosa
Blue green
43
False about P.aeruginosa lab diagnosis
a. Isolate on BAP or TCBS
b. Fruity (sweet, grape-like) odor and can grow at 42degrees Celsius
c. Forms smooth round colonies with fluorescent green color
d. Positive at lactose fermentation and oxidase
A
Eosin-methythionine blue agar dapat
44
Which is false?
A. Pyocyanin is produced by all Pseudomonas species
B. Pyoverdin is produced by P. aeruginosa
C. None of the above
A. Pyocyanin is produced by all Pseudomonas species
Not all
45
Which is not an effective antibiotic against P.aeruginosa?
A.Aztreonam
B. Imipenem
C. Penicillin
D. Ceftazidime
E. Cefeperazone
C
Ciprofloxacin dapat
46
False about Gardnerella vaginalis
(1) Small and pleomorphic
(2)gram-variable rod
(3)obligate anaerobe
(3)obligate anaerobe
Facultative dapat
47
Identify: A condition in women where normal balance of bacteria in the vagina is disrupted & replaced by an overgrowth of certain bacteria
Bacterial vaginosis
48
False about bacterial vaginosis
A.Foul or “fishy” vaginal odor
B.increased amount of gray-white milky discharge
C.Accompanied by pain, itching or burning
D.caused only by Gardnerella vaginalis
D
Polymicrobic infection siya
49
Which is not a direct condition for growth?
(1) Presence of Lactobacilli
(2) Change in pH of vaginal fluid
(3) Decreased acidity
(1)
Absence of Lactobacilli dapat
50
Which is not a direct risk factor for bacterial vaginosis?
(1) Changing sex partners
(2) Douching
(3) Intrauterine device for contraception
(4) none of the above
(4) none of the above
51
Identify the test used to detect bacterial vaginosis
a. sample + potassium hydroxide solution
b. positive result: release of amines, producing fishy odor
Amine/Whiff Test
52
True or false: When a more mixed flora is present and Lactobacillus is absent or present in low numbers, the smear would be diagnosed as consistent with bacterial vaginosis.
True
53
a. homogenous vaginal discharge
b. vaginal pH > 4.5
c.presence of clue cells (>20% of all epithelial cells)
d. fishy odor after addition of KOH to vaginal secretions
The aforementioned are collectively called __
Amsel criteria
54
True or false:"When the examination fits into the Amsel criteria, you need not culture”
True
55