Bacte Lab - Quiz 1 Flashcards
(48 cards)
– materials containing all essential nutrients for bacterial growth
- → for bacterial growth
- culture media may be in granular or powdered form
Culture media
-food and soil (where it is planted) for bacteria
Culture media
for how long and at what temperature must the CM have for the bacteria to grow
35 – 37C, 18 – 24 hours
2 forms of CM
granular or powdered form
CM for motility
Sulfide-Indole-Motility (SIM)
Characteristics of Good Culture Media
- Sterile
- Free of inhibitory substances
- Adequate amount of Salt, Water & Certain Essential Nutrients
- Has a proper pH
Classif of CM accdg to physical state or consistency
solid
semi-solid
liquid
Classif of CM accdg to physical state or consistency
no solidifying agent
liquid
Classif of CM accdg to physical state or consistency
routinely used for bacterial cultivation
liquid
sign of bacterial growth in liquid media
turbidity
example of liquid media
broth
Classif of CM accdg to physical state or consistency
with solidifying agent
solid
most common solidifying agent
agar
examples of solidifying agents
agar
albumin
gelatin
solidifying agent that is used as a diagnostic medium
*Gelatin
agar is liquid at what temp
100C
agar is solid at what temp
38C
Gelatin is solid at what temp
25C
agar concentration of solid media
1.5-3%
agar conc of semi-solid media
0.5 - 1%
Classif of CM accdg to physical state or consistency
used to determine if bacteria is motile or not
semi-solid
classif based on application or function
basal/ordinary/simple enriched selective differential assay medium maintenance w/o glucose
classif based on application or function
useful for assaying vitamins, antibiotics, amino acids
assay medium
classif based on application or function
with inhibitory substances
selective
