GPHT LAB - Apparatus I

Question 1

machine on which thin sections are cut

Answer 1

microtome

Question 2

process of using a microtome

Answer 2

microtomy

Question 3

principle of microtomy

Answer 3

pawl is brought into contact with a ratchet wheel, which is connected to a mill head micrometer screw. this action turns the ratchet wheel, which in turn rotates the screw. By this means the block is moved towards the knife at a predetermined thickness

Question 4

3 essential parts of a microtome

Answer 4

1) knife and knife carrier
2) block holder or chuck
3) ratchet feed wheel, adjustment screws, pawl

Question 5

An essential part of the microtome used for actual cutting of tissue sections

Answer 5

knife and knife carrier

Question 6

An essential part of the microtome where the tissue is held in position

Answer 6

block holder or chuck

Question 7

An essential part of the microtome used to line up the tissue block in proper position with the knife, adjust the proper thickness of the tissue for successive sections

Answer 7

ratchet feed wheel, adjustment screws, pawl

Question 8

An essential part of the microtome adjusted or manipulated during microtomy

Answer 8

ratchet feed wheel, adjustment screws, pawl

Question 9

types of microtome (5)

Answer 9

rotary (minot)
rocking 
freezing
ultra-thin
sliding or base sledge

Question 10

microtome invented by Caldwell Trefall

Answer 10

Rocking

Question 11

microtome invented by Queckett

Answer 11

Freezing

Question 12

microtome invented by Minot

Answer 12

Rotary (Minot)

Question 13

microtome invented in 1881

Answer 13

Rocking

Question 14

microtome invented in 1885 - 1886

Answer 14

Rotary (Minot)

Question 15

microtome invented in 1848

Answer 15

freezing

Question 16

microtome developed by Adams

Answer 16

Sliding or Base Sledge

Question 17

microtome developed in 1789

Answer 17

Sliding or Base Sledge

Question 18

microtome also known as Cambridge

Answer 18

Rocking

Question 19

Rocking Microtome also known as

Answer 19

Cambridge

Question 20

simplest of all microtomes

Answer 20

Rocking

Question 21

what type of microtome in which the tissue block being mounted on the end of a spring poised rocking arm, the knife held in horizontal position, blade face up and slightly inclined towards the block

Answer 21

Rocking

Question 22

disadvantage of rocking microtome

Answer 22

will not produce GOOD serial sections

Question 23

ribbons of how many sections are produced w/ a rocking microtome

Answer 23

60-90

Question 24

thickness of the sections produced by a rocking micrometer in um

Answer 24

10-12 um

Question 25

what type of microtome | relatively small length of knife is available for use; dangerously placed w/ the blade up

Answer 25

rotary (minot)

Question 26

what type of microtome | not suitable for cutting large blocks

Answer 26

rotary (minot)

Question 27

what type of microtome | for cutting serial sections

Answer 27

rotary (minot)

Question 28

what type of microtome | most commonly used; present in the lab

Answer 28

rotary (minot)

Question 29

what type of microtome | able to section paraffin-embedded sections

Answer 29

rotary (minot)

Question 30

what type of microtome consists of a central screw by which the block holder is perforated, attached to it is the food pipe carrying carbon dioxide from a cylinder

Answer 30

freezing

Question 31

in the freezing microtome, what allows the release of rapid intermittent burst of carbon dioxide

Answer 31

simple lever-operated valve

Question 32

in the freezing microtome, what is the propellant or freezing agent

Answer 32

carbon dioxide

Question 33

what type of microtome | used for rush frozen sections

Answer 33

freezing

Question 34

what type of microtome | capable of cutting sections at 0.5 micron required for EM

Answer 34

ultra-thin

Question 35

what type of microtome | knife contains selected fragments of broken plate glass

Answer 35

ultra-thin

Question 36

what knife can be used for ultra-thin microtome

Answer 36

special diamond knife

Question 37

what type of microtome | for cutting extremely hard blocks and for frozen sections

Answer 37

sliding or base sledge

Question 38

what type of microtome | first microtome invented

Answer 38

sliding or base sledge

Question 39

microtome knife is made of what?

Answer 39

stainless steel

Question 40

histology technician's too of his profession

Answer 40

microtome knife

Question 41

personal possession of histo tech

Answer 41

microtome knife

Question 42

every technician should be given at least __ good knives

Answer 42

2

Question 43

3 basic shapes of microtome knife

Answer 43

biconcave plane concave plane wedge

Question 44

length of biconcave

Answer 44

120 mm

Question 45

microtome knife used for paraffin sections with rocking or rotary type microtome

Answer 45

biconcave

Question 46

microtome knife that can be used on a carbon dioxide freezing microtome

Answer 46

biconcave

Question 47

microtome knife very useful for cutting paraffin tissue blocks

Answer 47

biconcave

Question 48

biconcave knife is found on what microtome

Answer 48

rotary