CM LEC - CSF up to CSF Protein Flashcards
(136 cards)
1
Q
CSF produced by
A
choroid plexus
2
Q
choroid plexus produces how many percent of CSF
A
70%
3
Q
where does the 30% CSF come from
A
plasma filtration
4
Q
provides nutrients to CNS
A
CSF
5
Q
channel for removal of waste products
A
CSF
6
Q
mechanical buffer
A
CSF
7
Q
protects brain and spinal cord from sudden pressure changes
A
CSF
8
Q
CSF is also called __
A
fluid under pressure
9
Q
volume regulator
A
CSF
10
Q
normal CSF volume baby
A
10-60 ml
11
Q
normal CSF volume adult
A
140 - 170 ml
12
Q
rate of formation
A
20 ml CSF/hour
13
Q
gold std for diagnosis of meningitis
A
CSF analysis
14
Q
3 layers of meninges
A
dura
arachnoid
pia mater
15
Q
types of inflammation
A
septic
aseptic
16
Q
type of inflammation
viral, bacterial cause or other infectious agents
A
septic
17
Q
types of inflammation
due to trauma
A
aseptic
18
Q
methods of CSF collection
A
lumbar, cisternal, ventricular puncture
19
Q
methods of CSF collection
w/in 3rd, 4th, 5th lumbar vertebrae
A
lumbar puncture
spinal puncture
lumbar tap
20
Q
methods of CSF collection
most common
A
lumbar puncture
spinal puncture
lumbar tap
21
Q
methods of CSF collection
used among infants only
done while baby’s fontanelle is open
A
ventricular puncture
22
Q
methods of CSF collection
at base of the skull, not popular
A
cisternal
23
Q
too much sx can’t be collected unless the pressure is
A
normal
24
Q
opening pressure for adult
A
90-180 mm H20
25
opening pressure for young
10 - 100 mm H20
26
2 materials mentioned, used for csf collection
L-shaped adaptor (where needle is attached)
| sterile manometer tube (measures pressure)
27
when can doctor aspirate?
when pressure is constant
28
closing pressure
45 -90 mm H20
29
when pressure suddenly drops
subarachnoid blockage
30
when pressure suddenly drops, do not ___
aspirate
31
determines the subarachnoid block which lowers the opening pressure
Queckenstendt test
32
CSF is always considered a STAT specimen except for vial # ?
2
33
vial # 1 is for what section
Chemistry/Serology
34
vial # 2 is for what section
Bacteriology
35
vial # 3 is for what section
Hematology
36
at what temp should vial # 1 be kept
freezing temp/freezer
37
at what temp should vial # 2 be kept
RT/incubator
38
at what temp should vial # 3 be kept
refrigerator temp
39
routine tests done on csf in chem section
CSF glucose
| CSF protein
40
special tests done on csf in chem section
```
CSF CK - MB
CSF CK -MT
CSF LDH
Glutamine
Lactate
```
41
tests done on csf in serology section
vDRL
FTA - ABS
CSF C-RP
42
vDRL meaning
veneral disease research laboratory
43
FTA-ABS meaning
Fluorescent Treponemal Antibody Absorption Test
44
tests done on csf in bacte section
G/S
C/S
AFB
India Ink
45
tests done on csf in hema section
WBC Count
| WBC Differential Count
46
RBC in CSF indication of
traumatic puncture
47
physical analysis is done on w/c section
hema
48
chemical analysis is done on w/c section
cc
49
microscopic analysis is done on w/c section
bacte
50
possible appearance of CSF (5)
```
crystal clear
cloudy, milky, turbid
xanthochromic
clotted
pellicle
```
51
normal appearance of csf
colorless and clear
52
CSF comparable to _
water
53
blank used for CSF analysis
distilled water
54
appearance of CSF
| indicates no cell count
clear
55
appearance of CSF
| indicates moderate cell count
cloudy/hazy
56
appearance of CSF
| indicates high cell count
turbid
57
purpose of dilution of sx prior to counting
so cells are not overlapping
58
appearance of CSF
| pale orange/ reddish/ yellowish discoloration
xanthochromic
59
xanthochromic sx has the presence of (4)
bilirubin, carotene, Hgb, protein
60
appearance of CSF
| indicates traumatic puncture
clotted
61
appearance of CSF
| tiny white aggregates on sample surface
pellicle
62
pellicle also described as __
cobweb or tigerweb
63
appearance of CSF
| correlated with TB meningitis
pellicle
64
specimen suspected with __ and __ should not be kept in fridge
H. influenzae and N. meningitis
65
specimen suspected with H. influenzae and N. meningitis
| because these are __
labile, killed at certain temp
66
Bloody specimen can have 2 causes
Traumatic aspiration
| True intracranial bleeding
67
distribution of RBCs in Traumatic aspiration
uneven
68
distribution of RBCs in True intracranial bleeding
even
69
Presence of clot in TA
+ due to plasma contamination
70
Presence of clot in TIB
-
71
Xanthochromia in TA
clear
72
Xanthochromia in TIB
xanthochromic
73
CSF CK-BB in TA
not mentioned
74
CSF CK-BB in TIB
higher
75
Isoenzymes of CK
CK - MM
CK - BB
CK - MB
76
CSF Lactate in TA
not mentioned
77
CSF Lactate in TIB
increased
78
when does lactate increase
during tissue destruction
79
transparency of csf
clear
80
sp gr of csf
1.006 - 1.008
81
pH of csf
7.30 - 7.35
82
WBC Count Normal Values for adult
0-5WBC/ ul
83
WBC Count Normal Values for infants
0-30 WBC/ ul
84
increased WBC in CSF
pleocytosis
85
if sx is clear
what is the dilution
manner of counting
where to count/ what formula to use
no dilution
direct counting
count in 9 large squares
86
if sx is hazy
what is the dilution
manner of counting
where to count/ what formula to use
1:10 dilution
count in 5 large squares
use std formula for actual wbc count
87
if sx is turbid
what is the dilution
manner of counting
where to count/ what formula to use
1:20
count in 4 large squares
use std formula for actual wbc count
88
diluting fluid for CSF WBC count
Tuerk's
| 3% Acetic Acid
89
what tube is used in charging for CSF WBC count
plain blue capillary tube
90
manner of mixing for CSF WBC count
inversion
91
for CSF WBC count, may be counted as a wbc
nucleated red cell
92
methods of obtaining sediment for diff count
sedimentation
filtration
cytocentrifugation
centrifugation
93
most common, most backward method of obtaining sediment for diff count
sedimentation
94
what to d with supernatant after sedimentation
give to chem section
95
stain used in CSF diff count
wright stain
96
advanced method of obtaining sediment for diff count
cytocentrifugation
97
in diff count, if cells counted do not reach 100, report only the ___ seen
number of cell types seen
98
method of obtaining sediment for diff count w/ good cell recovery
cytocentrifugation
99
method of obtaining sediment for diff count w/ complete cell recovery
filtration
100
stain for blood cells
wright
101
stain for cancer cells
papanicolaou
102
stain for parasitic cells
giemsa
103
CSF Cell constituents and %
70% lympho
| 30% mono
104
> 70% PMN indicates
bacteria meningitis
105
Early viral, fungal or tubercular meningitis demonstrates increase in what cells
PMN
106
Chronic viral, fungal or tubercular meningitis demonstrates increase in what cells
Lympho
107
parasitic meningitis demonstrates increase in what cells
eosino
108
bacterial meningitis demonstrates increase in what cells
PMN
109
Immature leukocytes indicates
leukemia
110
malignant cells indicates
tumor/cancer
111
plasma cells and reactive lympho indicates
HIV/ AIDS
112
macrophages in brain
microglial cells
113
bacteria that causes increase in lympho
Listeria monocytogenes
114
fungi with star burst appearance
C. neoformans
115
N.V. of CSF protein
15 - 45 mg/dL
116
Elevated CSF protein indicates
damage in BBB
117
why CSF protein is
CSF protein is a product of filtration, proteins cannot pass the filtering membrane
118
conditions of increased CSF protein
meningitis
traumatic tap
IgG synthesis
119
in traumatic tap, a drop of plasma causes how much increase of protein
10x
120
globulin produced during infection
IgG
121
conditions of decreased CSF protein
Elevated intracranial pressure
large sample removal
hyperthyroidism
otorrhea
122
CSF leaks from brain to ears
otorrhea
123
CSF leaks from brain to nose
rhinorrhea
124
protein fraction normally in csf (decreasing order)
```
Alb
Pre-alb
Ceruloplasmin
Transferrin
IgA
IgG
```
125
Marker to differentiate CSF from other body fluids
Tau protein or T protein or carbohydrate deficient protein (?)
126
proteins not normally in CSF
fibrinogen
IgM
beta lipoprotein
127
precipitating agents (2)
SSA
| TCA
128
SSA meaning
sulfosalicylic acid
129
TCA meaning
trichloroacetic acid
130
can ppt alb fraction better than glob fraction
SSA
131
can ppt alb fraction and glob fraction better
TCA
132
more sensitive ppt agent
TCA
133
Supernatant (Alb) measured using what dyes
BCG
MO
HABA
134
Used to visualize all protein fractions
electrophoresis
135
type of electrophoresis commonly used for CSF
PAGE
136
first choice dye
| has affinity for all protein fractions
Coomassie Brilliant Blue
