Pathology 1 - Intro Flashcards Preview

CJ: UoL Medicine Semester Two (ESA2) > Pathology 1 - Intro > Flashcards

Flashcards in Pathology 1 - Intro Deck (11):
1

Define 'disease'

A pathological condition of a body part, organ or system characterised by an identifiable group of signs/symptoms

2

What is autolysis?

Self-digestion of tissues that occurs when the blood supply is cut off

3

What are fixatives?

Chemicals which inactivate tissue enzymes and denature proteins, prevent bacterial growth and harden tissue

4

What is the most commonly used fixative?

Formalin (formaldehyde in water)

5

How are the tissue samples stored while they are fixed?

They are put in 'cassettes' which have holes in, then placed in racks in formalin

6

What is the tissue embedded in so that thin sections can be cut?

It is embedded in paraffin wax

7

How is paraffin wax inserted inside the cells?

- Dehydration using alcohol in a vacuum so water is drawn out of the cells
- replace alcohol with xylene which mixes with wax
- replace xylene with molten paraffin wax

8

What is used to cut very thin sections of tissue?

A microtome

9

What is the tissue usually stained with?

H&E

10

Give some examples of things that can be identified using immunohistochemistry

- Actin
- Cadherins
- Hormone receptors
- Her2 receptor
- Microorganisms

11

What are cytokeratins?

A family of intracellular fibrous proteins present in almost all epithelia. They can give info about the primary site of a carcinoma

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